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Pathology --- Pathology, Cellular --- Molecular biology --- Molecular biology. --- Pathology. --- Pathology, Cellular. --- Cellular pathology --- Cytopathology --- Disease (Pathology) --- Molecular biochemistry --- Molecular biophysics --- Pathologies --- Disease --- pathology --- Cytodiagnosis --- Medical sciences --- Diseases --- Medicine --- Medicine, Preventive --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology

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biochemie --- General biochemistry --- proteïnen --- Protein Conformation --- Proteins --- Protéines --- physiology --- Structure --- Conformation --- Protein Conformation. --- Proteins. --- physiology. --- Structure. --- Conformation. --- 547.96 --- 547.96 Proteins --- Protéines --- Protein conformation --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Algemene biochemie --- Proteins - physiology. --- Proteins - Structure. --- Proteins - Conformation. --- Acqui 2006 --- PROTEINS --- CONFORMATION --- PHYSIOLOGY --- STRUCUTRE

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Proteomics --- Proteins --- Medical genetics --- Protéomique --- Protéines --- Génétique médicale --- Periodicals. --- Périodiques --- Proteomics. --- Medical genetics. --- Proteins. --- Proteomica. --- Proteids --- Clinical genetics --- Diseases --- Heredity of disease --- Genetic aspects --- translational proteomics --- molecular medicine --- Biomolecules --- Polypeptides --- Molecular biology --- Human genetics --- Medical sciences --- Pathology --- Genetic disorders --- Genomics --- Health Sciences --- Life Sciences --- Genetics --- Medical Education, Training & Research --- Medical Informatics --- General and Others --- proteomics --- Human medicine

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In this new edition of the very successful Protein Purification Protocols (1996), Paul Cutler completely updates the existing protocols to reflect recent advances and adds an enormous new array of proteomic techniques for protein isolation and analysis. These cutting-edge techniques include not only two-dimensional gel electrophoresis for analysis and characterization, but also analytical chromatography for multidimensional separations of proteins and peptides, and mass spectrometry for isolating proteins. With the many recent advances in technology, simple spectrometric detection is no longer the only option for separating proteins, and the authors treat in full detail all the newer methods for these separations. Comprehensive and highly practical, Protein Purification Protocols, Second Edition, brings together all the key methodologies that both novice and experienced investigators need to carry out successful experimental work on proteins and their functions today.

Animal protein --- Protéine végétale --- Plant protein --- Extraction --- Purification --- Proteolysis --- Enzymes --- Chromatographie --- Chromatography --- Électrophorèse --- Electrophoresis --- Biomolecules. --- Electronic books. -- local. --- Proteins -- Purification -- Laboratory manuals. --- Proteins --- Publication Formats --- Amino Acids, Peptides, and Proteins --- Chemicals and Drugs --- Publication Characteristics --- Laboratory Manuals --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- 577.112.083 --- 57.088.3 --- 577.112 --- -Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Proteins--?.083 --- Methods and techniques for separation,fractionation and purification. --- -Laboratory manuals --- -Proteins--?.083 --- 577.112 Proteins --- 57.088.3 Methods and techniques for separation,fractionation and purification. --- 577.112.083 Proteins--?.083 --- -577.112 Proteins --- Proteids --- Methods and techniques for separation,fractionation and purification --- Biological molecules --- Molecules --- Molecular biology --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition

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The bacterium Escherichia coli – E. coli for short – has long been the organism of choice for unraveling biochemical pathways, deciphering the genetic code, learning how DNA is replicated and read, and even for manufacturing proteins of commercial interest. For some thirty years, it also has been a model for studying the molecular biology of behavior. E. coli swims in a purposeful manner, propelled by long thin helical filaments, each driven at its base by a reversible rotary engine. As a microscopic organism immersed in an aqueous environment, it has mastered physical constraints utterly different from any that we know, devising sensors, comparators, and motors on the nanometer scale. This cross-disciplinary monograph describes these feats in a manner accessible to scientists, engineers, and others not trained in microbiology who would like to learn more about living machines. It treats the history of the subject, the physiology, physics, biochemistry and genetics, largely from first principles. It is all about a small but remarkably sophisticated friend who lives in your gut. Topics discussed include: How does E. coli move about? How do cells decide whether life is getting better or worse? What is the machinery that makes this behavior possible? How is the construction of this machinery programmed? How does this machinery work? What remains to be discovered?

Escherichia coli --- Microbiologie --- Bacteriën --- Microorganisms -- Motility. --- Escherichia --- Enterobacteriaceae --- Gammaproteobacteria --- Gram-Negative Facultatively Anaerobic Rods --- Proteobacteria --- Life sciences. --- Medical microbiology. --- Molecular biology. --- Bacteriology. --- Biophysics. --- Biological physics. --- Life Sciences. --- Biophysics and Biological Physics. --- Medical Microbiology. --- Molecular Medicine. --- Escherichia coli. --- Microorganisms --- Motility. --- Microbiology. --- Medicine. --- Biological and Medical Physics, Biophysics. --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology --- Biological physics --- Biology --- Medical sciences --- Physics --- Microbiology --- Microorganisms. --- Germs --- Micro-organisms --- Microbes --- Microscopic organisms --- Organisms --- Bacteriën